产品编号:N112
产品描述:Anti-Human NAP-2 (Antigen Aff. Pur.)
反应种属:human
实验方法:ELISA, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 7 (CXCL7)
Antigen Affinity Purified
(PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1)
Polyclonal Antibody
Prod. No.: N112
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 7 (CXCL7) recognizes Human CXCL7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human CXCL7 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human CXCL7 is approximately 0.3 ng/well.
Western Blotting: To detect Human CXCL7 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CXCL7 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human NAP-2 (CXCL7) (Leinco Prod. No.: N119).
References
1. Poncz M et al. (1991) J Biol Chem. 266: 5785
2. Walz D et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su C et al. (1985) Biochemistry 24: 1762
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human NAP-2 (CXCL7) (Leinco Prod. No.: N119)
Anti-Human CXCL7 (Leinco Prod. No.: N139)
Recombinant Mouse CXCL7 (Leinco Prod. No.: T380)
Recombinant Rat CXCL7 (Leinco Prod. No.: T381)
Anti-Mouse CXCL7 (Leinco Prod. No.: C1380)
Anti-Mouse CXCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: C1478)
Anti-Mouse CXCL7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1523)
Anti-Human CXCL7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N124)
Recombinant Mouse Thymus Chemokine-1 (Leinco Prod. No.: T530)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
