产品编号:N143

产品描述:Anti-Human Nanog

反应种属:human

实验方法:ELISA, WB, ICC

标记:

规格:100ug

供应商:leinco

价格(RMB):5595.00

订购:

说明书:

Goat Anti-Human Nanog
Antigen Affinity Purified
Polyclonal Antibody

Prod. No.: N143
Host:        Goat
Pkg. Size: 1 mg, 100 µg
Storage:     -20°C to -70°C

Description
Specificity:
Rabbit Anti-Human Nanog recognizes Human Nanog. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Nanog, a homeobox transcription factor1, plays a key role in maintaining stem cell pluripotency by regulating the expression of genes critical to stem cell renewal and differentiation.2 In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). Nanog may be useful in the immunohistochemical diagnosis of tumors. It is expressed in germ cells of the fetus and in some germ cell tumors of the gonads3 and central nervous system.4

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human Nanog (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB

References
1. Schöler HR.et al. (2003) Cell 113: 551
2. Pei D. et al. (2005) J Biol. Chem. 280: 1401
3. Rajpert-De Meyts E. et al. (2005) Histopathology 47: 48
4. Ligon KL. et al. (2006) Am J Surg Pathol. 30: 1613

Related Products
Recombinant Human Nanog (Leinco Prod. No.: N140)
Anti-Human Nanog-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N144)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.