产品编号:M183
产品描述:Anti-Human MIP-3β
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Macrophage Inflammatory Protein-3 Beta (MIP-3β)
Antigen Affinity Purified
(CCL19, AMAC-1, Ckb7, Ck-Beta 7, DC-CK1, MIP4, PARC, SCYA18, MIP-3B, Exodus-3, EBI1-Ligand Chemokine (ELC))
Polyclonal Antibody
Prod. No.: M183
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Macrophage Inflammatory Protein-3 Beta (MIP-3β) recognizes Human MIP-3β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Macrophage inflammatory protein-3beta (MIP-3beta) is a non-glycosylated polypeptide and member of the B-chemokine (C-C) family of cytokines. It plays an important role in the inflammatory response of human T cells and tissue macrophages. MIP-3beta is a potential chemoattractant for endometrial natural killer cells.1 It may be important in trafficking of T cells in thymus, and T cell and B cell migration to secondary lymphoid organs.2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human MIP-3β (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.8 µg/ml. A suitable detection antibody is Anti-Human MIP-3β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M198) at a concentration of approximately 0.8 µg/ml. A suggested standard for this assay is Recombinant Human MIP-3β (Leinco Prod. No.: M168).
Neutralization: This antibody is useful for neutralization of Human MIP-3β bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MIP-3β (at 0.05 ng/ml) is 2 - 10 µg/ml.
Western Blotting: To detect Human MIP-3β this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human MIP-3β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human MIP-3β (Leinco Prod. No.: M168).
References
1. Honjo H. et al. (2004) Fertil Steril. 1: 876
3. Broxmeyer HE. et al. (1998) J Immunol. 160: 2418
Related Products
Anti-Human MIP-3β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M198)
Recombinant Human MIP-3β (Leinco Prod. No.: M168)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human MIP-3β (Leinco Prod. No.: M1094)
Recombinant Mouse MIP-3β (Leinco Prod. No.: M1106)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
