产品编号:M147

产品描述:Anti-Human MIP-1α

反应种属:human

实验方法:ELISA, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5325.00

订购:

说明书:

Goat Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α)
Antigen Affinity Purified
(G0S19-1, LD78Alpha, MIP1A, SCYA3, CCL3)
Polyclonal Antibody

Prod. No.: M147
Host:         Goat
Pkg. Size: 1 mg, 100 µg
Storage:     -20°C to -70°C

Description
Specificity:
Goat Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α) recognizes Human MIP-1α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human MIP-1α (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB

References
1. IB Pragnell et al. (1994) J. Biol. Chem. 269: 4974
2. DN Cook et al. (1996) J. of Leukocyte Biol. 59: 61
3. PA Ward et al. (1995) J of Immunol. 154: 4793

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.