产品编号:M176
产品描述:Anti-Human CCL22
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Chicken Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22)
Antigen Affinity Purified
(MDC, A-152E5.1, ABCD-1, DC/B-CK, MGC34554, SCYA22, STCP-1)
Polyclonal Antibody
Prod. No.: M176
Host: Chicken
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Chicken Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22) recognizes Human CCL22. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemoattract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once thes cells have migrated into tissue.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human CCL22 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti Chicken IgY/IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: C1177). The detection sensitivity of this indirect ELISA for Human MDC is approximately 0.3 ng/well.
Neutralization: This antibody is useful for neutralization of Human MDC bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MDC (at 10 ng/ml) is 0.4-2.0 µg/ml.
Western Blotting: To detect Human CCL22 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti Chicken IgY/IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: C1177) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CCL22 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human CCL22 (Leinco Prod. No.: M171).
References
1. Sozzani S. et al. (2001) Eur. J. Immunol. 31: 812
2. Elias CG. et al. (1998) J Immunol. 161: 5027
3. Gray PW. et al. (1997) J Exp Med. 185: 1604
4. Teixeira MM. et al. (2003) J Leuko Biol. 73: 356
Related Products
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TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human CCL22 (Leinco Prod. No.: M171)
Anti-Mouse CCL22-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1112)
Anti-Mouse CCL22 (Antigen Aff. Pur.) (Leinco Prod. No.: M1111)
Anti-Human CCL22 (Leinco Prod. No.: M1092)
Mouse CCL22 ELISA Development Kit (Leinco Prod. No.: M1113)
Anti-Human CCL22-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M129)
Recombinant Mouse CCL22 (Leinco Prod. No.: M202)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
