产品编号:L114
产品描述:Anti-Human Lymphotactin(Lptn)
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Lymphotactin (Lptn)
Antigen Affinity Purified
(SCYC1, LTN, LPTN, ATAC, SCM-1a, SCM-1, XCL1, Lptn)
Polyclonal Antibody
Prod. No.: L114
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Lymphotactin (Lptn) recognizes Human Lptn. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Lymphotactin (LTN) is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells.1 LTN plays an important role in maintaining the integrity of the epithelium and in mucosal immune responses.2 Lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection.
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human Lymphotactin
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human Lptn is approximately 0.3 ng/well.
Neutralization: This antibody is useful for neutralization of Human Lptn bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Lptn (at 0.5 ng/ml) is 20 - 30 µg/ml.
Western Blotting: To detect Human Lptn this polyclonal antibody can be used at a concentration of 1 - 2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Lptn is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Saucedo-Cardenas O. et al. (2009) Biotechnol. Lett. 31: 215
2. McGhee JR. et al. (1999) J. Immunol. 162: 1959
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human Lymphotactin (Leinco Prod. No.: L120)
Anti-Human Lymphotactin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L144)
Recombinant Mouse Lymphotactin (Leinco Prod. No.: L196)
Anti-Mouse Lymphotactin (Antigen Aff. Pur.) (Leinco Prod. No.: L212)
Anti-Mouse Lymphotactin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L229)
Anti-Mouse Lymphotactin (Leinco Prod. No.: X114)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
