产品编号:K122
产品描述:Anti-Human FGF-7
反应种属:human
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Rabbit Anti-Human Fibroblast Growth Factor 7 (FGF-7)
Antigen Affinity Purified
(HBGF-7, KGF)
Polyclonal Antibody
Prod. No.: K122
Host: Rabbit
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Fibroblast Growth Factor 7 (FGF-7) recognizes Human FGF-7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Fibroblast growth factor 7 (keratinocyte growth factor), also known as FGF7, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. FGF7 plays a significant role in the development of ovarian cancer.1
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human FGF-7/KGF (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.25 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 1 µg/ml with a suitable second step reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity of this indirect ELISA for Human FGF-7 is approximately 1 ng/well.
Western Blotting: To detect Human FGF-7 this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human FGF-7 is 1.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Ohtake Y. et al.(2005) Int. J. Oncol. 26: 1209
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human FGF-7 (Leinco Prod. No.: K123)
Anti-Human FGF-7 (Leinco Prod. No.: F1068)
Anti-Human FGF-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K127)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
