产品编号:I-506
产品描述:Anti-Human IRF3(CT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):6195.00
订购:
说明书:
Rabbit Anti-Human Interferon Regulatory Factor-3 (IRF3)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: I-506
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Interferon Regulatory Factor-3 (IRF3) recognizes an epitope near the C-terminus of Human and Mouse IRF3. This polyclonal antibody was purified using affinity chromatography.
Background:
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-α/β genes. IRF3 is present in an inactive form in the cytoplasm of most cells.2 Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1),3,4 whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA.3 IRF3 exists as at least two distinct isoforms.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
IRF3 (CT) Blocking Peptide (Leinco Prod. No.: I-518)
Blocking Peptide
IRF3 (CT) Blocking Peptide (Leinco Prod. No.: I-518)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse IRF3 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Ramos Whole Cell Lysate (Leinco Prod. No.: R1173).
References
1. Malmgaard, L. (2004) J. Interferon & Cyto. Res. 24:439
2. Au, W. C. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11657
3. Fitzgerald, K. A. et al. (2003) Nat. Immunol. 4:491
4. Sharma, S. et al. (2003) Science 300:1148
Related Products
IRF3 (CT) Blocking Peptide (Leinco Prod. No.: I-518)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Ramos Whole Cell Lysate (Leinco Prod. No.: R1173)
Anti-Human IRF3 (Intermediate Domain) (Leinco Prod. No.: I-507)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
