产品编号:I-366

产品描述:Anti-Human IL-20

反应种属:human

实验方法:ELISA, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5325.00

订购:

说明书:

Goat Anti-Human Interleukin-20 (IL-20)
Antigen Affinity Purified
(ZCYTO10, IL10D)
Polyclonal Antibody

Prod. No.: I-366
Host:           Goat
Pkg. Size: 1 mg, 100 µg
Storage:       -20°C to -70°C

Description
Specificity:
Goat Anti-Human Interleukin-20 (IL-20) recognizes Human IL-20. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Interleukin-20 (IL-20) is a pleiotropic inflammatory protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.1 IL-20 plays a critical role in the induction and maintenance of psoriasis.2

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-20 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human IL-20 is approximately 0.3 ng/well.
Western Blotting: To detect Human IL-20 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-20 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IL-20 (Leinco Prod. No.: I-338).

References
1. Kupper T.Set al. (2006) Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chem. 5: 243
2. Dam TM. et al. (2009) Br J Dermatol. 160: 284

Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-20 (Leinco Prod. No.: I-338)
Anti-Human IL-20-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-367)
Recombinant Mouse IL-20 (Leinco Prod. No.: I-526)
Anti-Mouse IL-20 (Leinco Prod. No.: I-623)
Human IL-20 ELISA Development Kit (Leinco Prod. No.: I-295)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.