产品编号:I-157
产品描述:Anti-Human IL-15
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Interleukin-15 (IL-15)
Antigen Affinity Purified
(IL-T, MGC9721)
Polyclonal Antibody
Prod. No.: I-157
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Interleukin-15 (IL-15) recognizes Human IL-15. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 15 (IL-15) is a novel cytokine that uses beta- and gamma-chain of IL-2R for signal transduction and is structurally similarity to IL-2. IL-15 is secreted by activated monocytes/macrophages following infection by virus.1 This cytokine induces cell proliferation of natural killer cells. This cytokine and interleukin 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. IL-15 can recruit and activate T lymphocytes in the synovial membrane, thereby contributing to Rheumatoid arthritis pathogenesis.2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-15 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human IL-15 is approximately 1 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3 - 10 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IL-15 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-15 (at 10 ng/ml) is 0.5 - 1.5 µg/ml.
Western Blotting: To detect Human IL-15 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-15 is 20 and 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IL-15 (Leinco Prod. No.: I-196).
References
1. Yoshikai Y.et al. (1996) J. Immunol. 156: 663
2. Liew FY. et al. (1996) Nat. Med. 4: 643
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-15 (Leinco Prod. No.: I-196)
Anti-Human IL-15-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-270)
Anti-Mouse IL-15 (Antigen Aff. Pur.) (Leinco Prod. No.: I-404)
Anti-Human IL-15 (Leinco Prod. No.: I-639)
Anti-Human IL-15-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-763)
Anti-Human IL-15 (Leinco Prod. No.: I-242)
Recombinant Rat IL-15 (Leinco Prod. No.: I-274)
Anti-Mouse IL-15-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-405)
Recombinant Mouse IL-15 (Leinco Prod. No.: I-280)
Recombinant Mouse IL-15 (Leinco Prod. No.: I-843)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
