产品编号:I-156
产品描述:Anti-Human IL-13
反应种属:human
实验方法:ELISA, FC, N, WB
标记:
规格:250ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Interleukin-13 (IL-13)
Antigen Affinity Purified
(ALRH, BHR1, MGC116786, P600, NC30)
Polyclonal Antibody
Prod. No.: I-156
Host: Goat
Pkg. Size: 1 mg, 250 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Interleukin-13 (IL-13) recognizes Human IL-13. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells1, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.1 The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-13 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.25 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, FC, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human IL-13 is approximately 2 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-13. A suggested method would be to stain cells expressing IL-13 with 3-10 µg per 1 x 106 cells in a total staining volume of 100 µl followed by Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641).
Western Blotting: To detect Human IL-13 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-13 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Wynn TA. et al. (2003) Annu Rev Immunol. 21: 425
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
