产品编号:I-391
产品描述:Anti-Human IL-9
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Interleukin-9 (IL-9)
Antigen Affinity Purified
(P40 Cytokine, T-Cell Growth Factor P40, HP40, P40 )
Polyclonal Antibody
Prod. No.: I-391
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Interleukin-9 (IL-9) recognizes Human IL-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 9, also known as IL9, is a TH2 cytokine1 belonging to the group of interleukins. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 stimulates the proliferation of a variety of hematopoietic lineages through its interaction with a receptor of the cytokine receptor superfamily.2 IL-9 plays a role in the complex pathogenesis of bronchial hyperresponsiveness as a risk factor for asthma.3
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-9 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<10 ng/mg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human IL-9 is approximately 0.3 ng/well.
Neutralization: This antibody is useful for neutralization of Human IL-9 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-9 (at 5 ng/ml) is 2-5 µg/ml.
Western Blotting: To detect Human IL-9 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-9 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IL-9 (Leinco Prod. No.: I-379).
References
1. Renauld JC. et al. (1995) J. Immunol 154: 5061
2. Yang YC. et al. (1995) J. Biol. Chem. 270: 20497
3. Levitt RC. et al. (1997) Proc Natl Acad Sci USA. 94: 13175
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-9 (Leinco Prod. No.: I-379)
Anti-Mouse IL-9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-394)
Recombinant Mouse IL-9 (Leinco Prod. No.: I-380)
Human IL-9 ELISA Kit (Leinco Prod. No.: I-493)
Anti-Mouse IL-9 (Antigen Aff. Pur.) (Leinco Prod. No.: I-393)
Anti-Human IL-9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-392)
Human IL-9 ELISA Development Kit (Leinco Prod. No.: I-828)
Recombinant Rat IL-9 (Leinco Prod. No.: I-842)
Anti-Rat IL-9 (Antigen Aff. Pur.) (Leinco Prod. No.: I-859)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
