产品编号:I-148

产品描述:Anti-Human IL-4

反应种属:human

实验方法:ELISA, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5325.00

订购:

说明书:

Goat Anti-Human Interleukin-4 (IL-4)
Antigen Affinity Purified
(BCGF, BCDF, B cell stimulating factor (BSF-1) )
Polyclonal Antibody

Prod. No.: I-148
Host:          Goat
Pkg. Size: 1 mg, 100 µg
Storage:       -20°C to -70°C

Description
Specificity:
Rabbit Anti-Human Interleukin-4 (IL-4) recognizes Human IL-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption.1 IL-4 is critical for inducing allergic responses.2 It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.3

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-4 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB

References
1. J Wesley Pike et al. (2001) PNAS 98: 2443
2. Fred D. Finkelman et al. (2003) J Immunol. 170: 3835
3. Kaplan MH et al. (2008) J Immunol. 180: 3551

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.