产品编号:G590
产品描述:Anti-Human Galectin-3(Gal-3)
反应种属:human
实验方法:ELISA, IHC-F, FC, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Galectin-3 (Gal-3)
Antigen Affinity Purified
(LGALS3, Galactose-Specific Soluble Lectin 3, Lectin Galactoside-Binding Soluble 3, IgE-Binding Protein, MAC2, L-29, CBP35)
Polyclonal Antibody
Prod. No.: G590
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.1 It is secreted from activated macrophages, basophils, and mast cells.2 Galectin-3 may play an important role in the acute phase of human atopic dermatitis.
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Rabbit Galectin-3 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), FC, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-0.1 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human Gal-3 is approximately 1 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Gal-3. A suggested method would be to stain cells expressing Gal-3 with 50 µg per 1 - 2.5 x 105 cells in a total staining volume of 200 µl followed by Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641).
References
1. Eisenthal A et al. (2009) Pathol Res Pract.
2. Dahlgren C. et al. (1998) Blood 91: 3430
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641)
Anti-Human Galectin-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G591)
Recombinant Human Galectin-3 (Leinco Prod. No.: G589)
Anti-Human Galectin-3 (Leinco Prod. No.: G716)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
