产品编号:F107
产品描述:Anti-Human FGF-β
反应种属:human
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Fibroblast Growth Factor-basic (FGF-Basic)
Antigen Affinity Purified
(B-FGF, FGF-2, FGF-β, FGFB, Prostatropin, NUDT6)
Polyclonal Antibody
Prod. No.: F107
Host: Goat
Pkg. Size: 1.0 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Fibroblast Growth Factor-basic (FGF-Basic) recognizes Human FGF-Basic. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Acidic and basic fibroblast growth factors (FGFs) are members of a family of proteins that exert pleiotropic effects in a range of cell types including skeletal myocytes.1 Fibroblast growth factor basic (FGF basic) significantly promote angiogenesis; a central event for the growth and metastasis of solid tumors.2 It is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. FGF-basic plays a central role during prenatal development and postnatal growth and regeneration of tissues, by promoting cellular proliferation and differentiation. FGF-basic is one of the angiogenesis factors and may promote wound healing.3
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human FGF-basic (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
References
1. Swain JL et al. (1991) Developement 111: 741
2. Grevers G et al. (1997) Laryngorhinootologie 76: 421
3. (2007) ORG Genetics
Related Products
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Recombinant Mouse FGF-Basic (Leinco Prod. No.: F1022)
Recombinant Rat FGF-Basic (Leinco Prod. No.: F1027)
Anti-Human FGF-Basic (Leinco Prod. No.: F149)
Anti-Human FGF-Basic-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F134)
Human FGF-Basic ELISA Development Kit (Leinco Prod. No.: F167)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
