产品编号:F106
产品描述:Anti-Human FGF-1
反应种属:human
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human FGF Acidic
Antigen Affinity Purified
(FGF-1, ECGF, HBGF-1, AFGF, ECGF-Beta, ECGFA, ECGFB, FGF-Alpha, FGFA, GLIO703)
Polyclonal Antibody
Prod. No.: F106
Host: Goat
Pkg. Size: 1.0 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human FGF Acidic recognizes Human FGF Acidic. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Fibroblast growth factor 1 (acidic), also known as FGF1, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
FGF-1 is the only member of the FGF family that binds with high affinity to all four FGF receptors.1
FGF1 plays an important role in hematopoietic stem cell homeostasis. The FGF pathway regulates primitive hematopoiesis by modulating transcription factors such as Gata1 expression level and activity.2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human FDF-acidic (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human FDF-Acidic is approximately 0.13 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 1.7 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human FDF-Acidic bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human FDF-Acidic (at 0.75 ng/ml) is 0.25 - 0.75 µg/ml.
Western Blotting: To detect Human FGF Acidic this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human FGF Acidic is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human FGF-Acidic (Leinco Prod. No.: E193).
References
1. Otlewski J. et al. (2009) Acta Crystallogr D Biol Crystallogr. 65: 67
2. Wang HY.et al. (2008) Zhonghua Xue Ye Xue Za Zhi 29: 535
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human FGF-Acidic (Leinco Prod. No.: E193)
Anti-Human FGF-Acidic-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F133)
Recombinant Human FGF Acidic (Leinco Prod. No.: F112)
Anti-Human FGF Acidic (Leinco Prod. No.: F1065)
Recombinant Mouse FGF-Acidic (Leinco Prod. No.: F1026)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
