产品编号:E116
产品描述:Anti-Human CCL21
反应种属:human
实验方法:ELISA, IHC-F, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Chemokine (C-C Motif) Ligand 21 (CCL21)
Antigen Affinity Purified
(6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Exodus-2)
Polyclonal Antibody
Prod. No.: E116
Host: Goat
Pkg. Size: 1.0 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 21 (CCL21) recognizes Human CCL21. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7.
In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect.
Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human CCL21 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human CCL21 is approximately .016 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human CCL21 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL21 (at 50 ng/ml) is 1 - 4 µg/ml.
Western Blotting: To detect Human CCL21 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CCL21 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse CCL21 (Leinco Prod. No.: E114)
Anti-Mouse CCL21 (Antigen Aff. Pur.) (Leinco Prod. No.: E115)
Anti-Human Exodus-2/CCL21-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E127)
Anti-Mouse CCL21-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E128)
Recombinant Human CCL21 (Mucin) (Leinco Prod. No.: C1365)
Anti-Human CCL21 (Leinco Prod. No.: C1407)
Anti-Mouse CCL21 (Leinco Prod. No.: C1421)
Anti-Mouse CCL21-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1500)
Recombinant Human Exodus-2 (CCL21) (Leinco Prod. No.: E113)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
