产品编号:E138
产品描述:Anti-Human Eotaxin-3
反应种属:human
实验方法:ELISA, IHC-F, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Eotaxin-3
Antigen Affinity Purified
(SCYA26, MIP-4 Alpha, Eotaxin-3, IMAC, MIP-4a, TSC-1, CCL26)
Polyclonal Antibody
Prod. No.: E138
Host: Goat
Pkg. Size: 1.0 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Eotaxin-3 recognizes Human Eotaxin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Eotaxin-3/CCL-26 belongs to a family of CC chemokines, which are potent chemoattractants for eosinophils that signal through the CCR3 receptor. 1 It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. It has been reported that eotaxin-3 is an antagonist for CCR2.2 Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Eotaxin-3 is up-regulated in the context of a T helper 2 mediated inflammatory bowel disease via the signal transducer and activator of transcription 6, thus suggesting that the intestinal epithelium actively participates in the recruitment of eosinophils at the site of inflammation.1
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human Eotaxin-3/CCL26 (aa 24 - 94)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human Eotaxin-3 is approximately 1.2 ng/well.
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 15 µg/ml.
Neutralization: This antibody is useful for neutralization of Human Eotaxin-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Eotaxin-3 (at 1 ng/ml) is 10 - 25 µg/ml.
Western Blotting: To detect Human Eotaxin-3 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Eotaxin-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Cuber JC.et al. (2005) Int J Biochem Cell biol. 37: 2559
2. Gerber B. et al. (2004) J Biol Chem. 279: 23357
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Eotaxin-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E126)
Anti-Human Eotaxin-3 (Leinco Prod. No.: E140)
Recombinant Human Eotaxin-3 (Leinco Prod. No.: E118)
Human Eotaxin-3 ELISA Development Kit (Leinco Prod. No.: E145)
Recombinant Human Eotaxin-3 (Leinco Prod. No.: E313)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
