产品编号:C848
产品描述:Anti-Human CXCL16
反应种属:human
实验方法:ELISA, IHC-F, FC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 16 (CXCL16)
Antigen Affinity Purified
(SCYB16, SR-PSOX, CXCLG16, Zmynd15, AV290116, BB024863, 0910001K24Rik)
Polyclonal Antibody
Prod. No.: C848
Host: Goat
Pkg. Size: 1.0 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 16 (CXCL16) recognizes Human CXCL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a transmembrane protein that facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation.1 CXCL16 is found in liver tissue2 and influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides.3 CXCL16 may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease.4
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human CXCL16
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 100 µg/ml mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), FC, N, WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3-10 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL16. A suggested method would be to stain cells expressing CXCL16 with 3-10 µg per 1 x 106 cells in a total staining volume of ≤100 µl followed by Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641).
Neutralization: This antibody is useful for neutralization of Human CXCL16 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL16 (at 20 ng/ml) is 0.2-0.7 µg/ml.
Western Blotting: To detect Human CXCL16 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CXCL16 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Mummidi S et al. (2004) J Biol Chem. 279: 3188
2. Adams DH et al. (2005) J Immunol. 174: 1055
3. Clinman DM et al. (2006) J Immunol. 177: 1575
4. Broedl UC et al. (2008) Scand J Gastroenterol. 43: 283
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse CXCL16 (Leinco Prod. No.: C847)
Anti-Human CXCL16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C849)
Anti-Mouse CXCL16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C452)
Recombinant Human CXCL16 (Leinco Prod. No.: C843)
Anti-Mouse CXCL16 (Antigen Aff. Pur.) (Leinco Prod. No.: C451)
Recombinant Human CXCL16 (Extracellular Domain) (Leinco Prod. No.: C1335)
Anti-Mouse CXCL16 (Leinco Prod. No.: C1430)
Anti-Mouse CXCL16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1505)
Human CXCL16 ELISA Development Kit (Leinco Prod. No.: C337)
Recombinant Mouse CXCL16 (Extracellular Domain) (Leinco Prod. No.: C1590)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
