产品编号:C385
产品描述:Anti-Human Cardiotrophin-1(CT-1)
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Cardiotrophin-1 (CT-1)
Antigen Affinity Purified
(CTF1)
Polyclonal Antibody
Prod. No.: C385
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Cardiotrophin-1 (CT-1) recognizes Human CT-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Antigen Distribution:
Human CT-1 is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lungs.
Background:
Human CT-1 is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence.
Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity.
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human Cardiotrophin-1
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human CT-1 is approximately 0.5 ng/well.
Neutralization: This antibody is useful for neutralization of Human CT-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CT-1 (at 15 ng/ml) is 0.1 - 0.3 µg/ml.
Western Blotting: To detect Human CT-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CT-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse Cardiotrophin-1 (Antigen Aff. Pur.) (Leinco Prod. No.: C1378)
Anti-Human Cardiotrophin-1 (Leinco Prod. No.: C1388)
Anti-Mouse Cardiotrophin-1 (Leinco Prod. No.: C1415)
Anti-Mouse Cardiotrophin-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1498)
Recombinant Human Cardiotrophin-1 (Leinco Prod. No.: C330)
Recombinant Mouse Cardiotrophin-1 (Leinco Prod. No.: C842)
Anti-Human Cardiotrophin-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C395)
Anti-Human CT-1 (Leinco Prod. No.: C1603)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
