产品编号:R1001
产品描述:Anti-Mouse RIP3
反应种属:mouse, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Mouse Receptor Interacting Protein 3 (RIP3)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: R1001
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Mouse Receptor Interacting Protein 3 (RIP3) recognizes Mouse and Rat RIP3. This polyclonal antibody was purified using affinity chromatography.
Background:
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3.1-3 RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.2,3
Known Species Cross Reactivity: Ms+, Rt+
Immunogen
Receptor Interacting Protein 3 Blocking Peptide (Leinco Prod. No.: R1008)
Blocking Peptide
Receptor Interacting Protein 3 Blocking Peptide (Leinco Prod. No.: R1008)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Mouse and Rat RIP3 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).
References
1. Yu, P. W. et al. (1999) Curr. Biol. 9(10):539
2. Sun, X. et al. (1999) J. Biol. Chem. 274(24):16871
3. Pazdernik, N. J. et al. (1999) Mol. Cell Bio. 19(10):6500
Related Products
Receptor Interacting Protein 3 Blocking Peptide (Leinco Prod. No.: R1008)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
