产品编号:R1230
产品描述:Anti-Mouse Raptor(CT)
反应种属:mouse
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Mouse Raptor
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: R1230
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Mouse Raptor recognizes an epitope near the C-terminus of Mouse Raptor. This polyclonal antibody was purified using affinity chromatography.
Background:
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation.3 These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels.4 Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.
Known Species Cross Reactivity: Ms+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Mouse Raptor by Western blot analysis at a concentration of 2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is L1210 Whole Cell Lysate (Leinco Prod. No.: L160).
References
1. Shamji, A. F. et al. (2003) Mol. Cell 12:271
2. Fingar, D. C. et al. (2004) Oncogene 23:3151
3. Yonezawa, K. et al. (2004) Biophys. Res. Commun. 313:437
4. Hara, K. et al. (1998) J. Biol. Chem. 273:14484
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
L1210 Whole Cell Lysate (Leinco Prod. No.: L160)
Anti-Human Raptor (Intermediate Domain) (Leinco Prod. No.: R1231)
Anti-Human Raptor (NT) (Leinco Prod. No.: R1232)
Raptor (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: R1236)
Raptor (NT) Blocking Peptide (Leinco Prod. No.: R1237)
Raptor (CT) Blocking Peptide (Leinco Prod. No.: R1241)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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