产品编号:I-299
产品描述:Anti-Mouse ICAD(CT)
反应种属:mouse
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Mouse ICAD
Purified No Carrier Protein
(DFF45, Inhibitor of Caspase-Activated DNASE (CAD))
Polyclonal Antibody
Prod. No.: I-299
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Mouse ICAD recognizes an epitope near the C-terminus of mouse ICAD. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.
Known Species Cross Reactivity: Ms+
Immunogen
ICAD (CT) Blocking Peptide (Leinco Prod. No.: I-311)
Blocking Peptide
ICAD (CT) Blocking Peptide (Leinco Prod. No.: I-311)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Mouse ICAD by Western blot analysis at a dilution of 1:500-1:2,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PN:M1142.
References
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184
2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
4. Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21 (RD1299)
Related Products
ICAD (CT) Blocking Peptide (Leinco Prod. No.: I-311)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PN:M1142
Anti-Mouse ICAD (NT) (Leinco Prod. No.: I-312)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
