产品编号:T292
产品描述:Anti-Human TSC1
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Tuberous Sclerosis Complex 1(TSC1)
Purified No Carrier Protein
(Hamartin)
Polyclonal Antibody
Prod. No.: T292
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Tuberous Sclerosis Complex 1 (TSC1) recognizes an epitope in the intermediate domain of Human, Mouse and Rat TSC1. This polyclonal antibody was purified using affinity chromatography.
Background:
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high.3 Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro,4 suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth.4 A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat TSC1 by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is EL4 Whole Cell Lysate (Leinco Prod. No.: E153).
References
1. Shamji, A. F. et al. (2003) Mol. Cell 12:271
2. Inoki, K. et al. (2005) Mol. Biol. Rev. 69:79
3. Tabancay, Jr., A. P. et al. (2003) J. Biol. Chem. 278:39921
4. Inoki, K. et al. (2003) Genes Dev. 17:1829
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
EL4 Whole Cell Lysate (Leinco Prod. No.: E153)
Anti-Human Tuberous Sclerosis Complex 1 (CT) (TSC1) (Leinco Prod. No.: T291)
Tuberous Sclerosis Complex 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T320)
Tuberous Sclerosis Complex 1 (CT) Blocking Peptide (Leinco Prod. No.: T321)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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