产品编号:T289
产品描述:Anti-Human TRPC6(CT)
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Transient Receptor Potential Channel 6 (TRPC6)
Purified No Carrier Protein
(Short Transient Receptor Potential Channel 6, Htrp6)
Polyclonal Antibody
Prod. No.: T289
Host: Rabbit
Conc.: 1.0 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Transient Receptor Potential Channel 6 (TRPC6) recognizes an epitope near the C-terminus of Human, Mouse and Rat TRPC6. This polyclonal antibody was purified using affinity chromatography.
Background:
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the “canonical TRP” (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types.1 Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, TRPC6 acts as a non-selective store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that TRPC6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human TRPC6 this polyclonal antibody can be used at a concentration of 0.5 - 2.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Mouse Lung Tissue Lysate (Leinco Prod. No.: M1014).
References
1. Contell, C. et al. (2002) Mol. Cell 9:229
2. Dietrich, A. et al. (2005) Pflugers Arch. 451:72
3. Trebak, M. et al. (2003) Cell Calcium 33:451
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Mouse Lung Tissue Lysate (Leinco Prod. No.: M1014)
Anti-Human Transient Receptor Potential Channel 6 (NT) (TRPC6) (Leinco Prod. No.: T290)
Transient Receptor Potential Channel 6 (NT) Blocking Peptide (Leinco Prod. No.: T318)
Transient Receptor Potential Channel 6 (NT) Blocking Peptide (Leinco Prod. No.: T319)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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