产品编号:T308

产品描述:Anti-Human Toso

反应种属:human

实验方法:WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Rabbit Anti-Human Toso
Purified No Carrier Protein
(Regulator of Fas-Induced Apoptosis, Fas Apoptotic Inhibitory Molecule 3, FAIM3)
Polyclonal Antibody

Prod. No.: T308
Host:         Rabbit
Conc.:       0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human Toso recognizes Human Toso. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis is an important process by which normal tissue homeostasis and function are maintained. One of the major signals that regulate this process is mediated by the activation of the Fas receptor by its ligand. This leads to the formation of a Fas-associated death domain (FADD)- containing death-inducing signaling complex and the activation of caspase-8, which in turn activates downstream effector caspases, such as caspase-3 and -7. Recent experiments have shown that overexpression of Toso, a novel regulator of Fas-induced apoptosis in lymphoid cells, in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex. Despite its predicted molecular weight, Toso often migrates at 60 kDa in SDS-PAGE.

Known Species Cross Reactivity: Hu+
Immunogen
Toso Blocking Peptide (Leinco Prod. No.: T324)
Blocking Peptide
Toso Blocking Peptide (Leinco Prod. No.: T324)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB, In vivo Depletion
Western Blotting: This antibody can be used to detect Human Toso by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Lung Tissue Lysate (Leinco Prod. No.: H1406).

References
1. Curtin, J. F. et al. (2003) Cell Signal. 15:983
2. Hitoshi, Y. et al. (1998) Immunity 8:461
3. Song, Y. et al. (2005) J. Biol. Chem. 280:9618
Related Products
Toso Blocking Peptide (Leinco Prod. No.: T324)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Lung Tissue Lysate (Leinco Prod. No.: H1406)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.