产品编号:T361

产品描述:Anti-Human TIRP(CT-1)

反应种属:human

实验方法:IHC-F, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human TIRP
Purified No Carrier Protein
(TRAM, TICAM2)
Polyclonal Antibody

Prod. No.: T361
Host:          Rabbit
Conc.:       0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human TIRP recognizes an epitope near the C-terminus of Human TIRP. This polyclonal antibody was purified using affinity chromatography.

Background:
TIRP is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TIRP, along with other molecules such as TRIF, MAL, and MyD88, serves as an adaptor protein that allows for the interaction and activation of the IL-1R-associated kinase (IRAK) family, the subsequent activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-?B (5). Expression of TIRP appears to be essential for TLR4 signalling (6).

Known Species Cross Reactivity: Hu+
Immunogen
TIRP (CT-1) Blocking Peptide (Leinco Prod. No.: T366)
Blocking Peptide
TIRP (CT-1) Blocking Peptide (Leinco Prod. No.: T366)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect Human TIRP by Western blot analysis at a concentration of 0.5 - 2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PC-3 Whole Cell Lysate (Leinco Prod. No.: P166).

References
1. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1
receptor adaptor family grows to five members. Trends in Imm.
2003;24:286-9.

2. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential
adapter utilization by toll-like receptors mediates TLR-specific
patterns of gene expression. Mol. Interv. 2003;3:466-77.

3. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu.
Rev. Immunol. 2003;21:335-76.

4. Janeway CA Jr and Medzhitov R. Innate immune recognition.
Annu. Rev. Immunol. 2002;20:197-216.

5. Bin L-H, Xu L-G, and Shu H-B. TIRP, a novel toll/interleukin-1
receptor (TIR) domain-containing adaptor protein involved in TIR
signaling. J. Biol. Chem. 2003;278:24526-24532.

6. Fitzgerald KA, Rowe DC, Barnes, BJ et al. LPS-TLR4 signaling
to IRF-3/7 and NF-kB involves the toll adaptors TRAM and TRIF.
J. Exp. Med. 2003;198:1043-1055.
(06-01D)
Related Products
TIRP (CT-1) Blocking Peptide (Leinco Prod. No.: T366)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PC-3 Whole Cell Lysate (Leinco Prod. No.: P166)
Anti-Human TIRP (CT-2) (Leinco Prod. No.: T360)
TIRP (CT-2) Blocking Peptide (Leinco Prod. No.: T365)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.