产品编号:T359

产品描述:Anti-Human TIRAP

反应种属:human

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human TIRAP
Purified No Carrier Protein
(MAL, TIR Domain Containing Adaptor Protein)
Polyclonal Antibody

Prod. No.: T359
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C

Description
Specificity:
Rabbit Anti-Human TIRAP recognizes an epitope in the intermediate domain of Human TIRAP. This polyclonal antibody was purified using affinity chromatography.

Background:
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors (4,5). In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling (6). Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-κB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling (6,7).

Known Species Cross Reactivity: Hu+
Immunogen
TIRAP (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T364)
Blocking Peptide
TIRAP (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T364)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect Human TIRAP by Western blot analysis at a concentration of 4 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is MCF7 Whole Cell Lysate (Leinco Prod. No.: M1123).

References
1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential
adapter utilization by toll-like receptors mediates TLR-specific
patterns of gene expression. Mol. Interv. 2003; 3:466-77.

2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu.
Rev. Immunol. 2003; 21:335-76.

3. Janeway CA Jr and Medzhitov R. Innate immune recognition.
Annu. Rev. Immunol. 2002; 20:197-216.

4. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1
receptor adaptor family grows to five members. Trends in Imm.
2003; 24:286-9.

5. McGettrick AF and O’Neill LAJ. The expanding family of
MyD88-like adaptors in Toll-like receptor signal transduction. Mol
Imm. 2004; 41:577-82.

6. Yamamoto Y, Sato S, Hemmi H, et al. Essential role for TIRAP
in activation of the signalling cascade shared by TLR2 and TLR4.
Nature 2002; 420:324-9.

7. Hoorng T, Barton GM, FlavellRA, et al. The adaptor molecule
TIRAP provides signalling specificity for Toll-like receptors.
Nature 2002; 420:329-333.
Related Products
TIRAP (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T364)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
MCF7 Whole Cell Lysate (Leinco Prod. No.: M1123)
Anti-Mouse TIRAP (CT) (Leinco Prod. No.: T358)
TIRAP (CT) Blocking Peptide (Leinco Prod. No.: T363)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.