产品编号:T276
产品描述:Anti-Human TAK1
反应种属:mouse, human, rat
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human TGF-β Activated Protein Kinase 1 (TAK1)
Purified No Carrier Protein
(MAP3K7)
Polyclonal Antibody
Prod. No.: T276
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human TGF-β Activated Protein Kinase 1 (TAK1) recognizes Human, Mouse and Rat TAK1. This polyclonal antibody was purified using affinity chromatography.
Background:
TAK1 (TGF-β; activated protein kinase 1) is a widely expressed enzyme originally identified as a mitogen-activated protein kinase kinase kinase (MAP3K7).1 It has since been shown to mediate various intracellular signaling pathways, such as those induced by TGF-Β and members of the Toll-IL-1R (TIR) superfamily,2 thus acting as an intermediate in both proliferative and innate and adaptive immune responses.3 TAK1 is normally present in cells in a complex with TAK1-binding protein 1 (TAB1) and either TAB2 or TAB3.4 Association with TAB1 triggers TAK1 kinase activity by inducing TAK1 autophosphorylation, while TAB2 and possibly TAB3 may contribute to SAPK2a/p38a-mediated feedback inhibition of TAK1 activity.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
TGF-β Activated Protein Kinase 1 (NT) Blocking Peptide (Leinco Prod. No.: T303)
Blocking Peptide
TGF-β Activated Protein Kinase 1 (NT) Blocking Peptide (Leinco Prod. No.: T303)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat TAK1 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Rat Thymus Tissue Lysate (Leinco Prod. No.: R1172).
References
1. Yamaguchi, K. et al. (1995) Science 270:2008
2. Irie, T. et al. (2000) FEBS Lett. 467:160
3. Akira, S. et al. (2004) Nat. Rev. Immunol. 4:499
4. Jiang, Z. et al. (2002) Mol. Cell Biol. 22:7158
Related Products
TGF-β Activated Protein Kinase 1 (NT) Blocking Peptide (Leinco Prod. No.: T303)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Rat Thymus Tissue Lysate (Leinco Prod. No.: R1172)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
