产品编号:I-325
产品描述:Anti-Human IKKα(CT-1)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human IκB Kinase Alpha (IKKα)
Purified No Carrier Protein
(IKK-1)
Polyclonal Antibody
Prod. No.: I-325
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human IκB Kinase Alpha (IKKα) recognizes an epitope near the C-terminus of Human IKKα. This polyclonal antibody was purified using affinity chromatography.
Background:
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories.1-4 IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKβ). IKKa and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-α. IKKα is expressed in variety of human tissues.
Known Species Cross Reactivity: Hu+
Immunogen
IKKα (CT-1) Blocking Peptide (Leinco Prod. No.: I-313)
Blocking Peptide
IKKα (CT-1) Blocking Peptide (Leinco Prod. No.: I-313)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human IKKα by Western blot analysis at a dilution of 1:1000-1:2000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are HL60 Whole Cell Lysate (Leinco Prod. No.: H1403) and Jurkat Whole Cell Lysate (Leinco Prod. No.: J103).
References
1. DiDonato, J. A. et al. (1997) Nature 388:548
2. Regnier, C. H. et al. (1997) Cell 90:373
3. Zandi, E,. et al. (1997) Cell 91:243
4. Woronicz, J. D. et al. (1997) Science 278:866
Related Products
IKKα (CT-1) Blocking Peptide (Leinco Prod. No.: I-313)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HL60 Whole Cell Lysate (Leinco Prod. No.: H1403)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
IKKα (CT-2) Blocking Peptide (Leinco Prod. No.: I-314)
IKKα (CT-3) Blocking Peptide (Leinco Prod. No.: I-315)
Anti-Human IκB Kinase Alpha (CT-2) (IKKα) (Leinco Prod. No.: I-300)
Anti-Human IκB Kinase Alpha (CT-3) (IKKα) (Leinco Prod. No.: I-301)
IκB Kinase Alpha (CT-2) (IKKα) Blocking Peptide (Leinco Prod. No.: I-469)
IκB Kinase Alpha (CT-1) (IKKα) Blocking Peptide (Leinco Prod. No.: I-470)
IκB Kinase Alpha (CT-3) (IKKα) Blocking Peptide (Leinco Prod. No.: I-471)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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