产品编号:I-300
产品描述:Anti-Human IKKα(CT-2)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human IκB Kinase Alpha (IKKa)
Purified No Carrier Protein
(IKK-1: Inhibitor of Nuclear Factor Kappa-B Kinase Alpha Subunit, NFκB Inhibitor Kinase Alpha)
Polyclonal Antibody
Prod. No.: I-300
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human IκB Kinase Alpha (IKKa) recognizes an epitope near the C-terminus of Human IKKa. This polyclonal antibody was purified using affinity chromatography.
Background:
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and bacteria product LPS. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories.1-4 IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKΒ). IKKα and IKKb interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-Α.IKKα is expressed in a variety of human tissues.
Known Species Cross Reactivity: Hu+
Immunogen
IKKα (CT-2) Blocking Peptide (Leinco Prod. No.: I-314)
Blocking Peptide
IKKα (CT-2) Blocking Peptide (Leinco Prod. No.: I-314)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human IKKa by Western blot analysis at a dilution of 1:500-1:1000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).
References
1. DiDonato, J. A. et al. (1997) Nature 388:548
2. Regnier, C. H. et al. (1997) Cell 90:373
3. Zandi, E,. et al. (1997) Cell 91:243
4. Woronicz, J. D. et al. (1997) Science 278:866
Related Products
IKKα (CT-2) Blocking Peptide (Leinco Prod. No.: I-314)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
IKKα (CT-1) Blocking Peptide (Leinco Prod. No.: I-313)
IKKα (CT-3) Blocking Peptide (Leinco Prod. No.: I-315)
Anti-Human IκB Kinase Alpha (CT-1) (IKKα) (Leinco Prod. No.: I-325)
Anti-Human IκB Kinase Alpha (CT-3) (IKKα) (Leinco Prod. No.: I-301)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
.jpg)
.jpg)
