产品编号:H1458
产品描述:Anti-Human Hrk
反应种属:mouse, human, rat
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Hrk
Purified No Carrier Protein
(Harakiri, Neuronal Death Protein DP5)
Polyclonal Antibody
Prod. No.: H1458
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Hrk recognizes mouse, rat and human Hrk. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Hrk Blocking Peptide (Leinco Prod. No.: H1460)
Blocking Peptide
Hrk Blocking Peptide (Leinco Prod. No.: H1460)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human Hrk by Western blot analysis at a concentration of 2.5-5.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Pancreas Tissue Lysate (Leinco Prod. No.: P187).
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Imaizumi K, Tsuda M, Imai Y, et al. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. J. Biol. Chem. 1997; 272:18842-8.
3. Inohara N, Ding L, Chen S, et al. harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-XL. EMBO J. 1997; 16:1686-94.
Related Products
Hrk Blocking Peptide (Leinco Prod. No.: H1460)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Pancreas Tissue Lysate (Leinco Prod. No.: P187)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
