产品编号:G620
产品描述:Anti-Human GITRL
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human GITRL
Purified No Carrier Protein
(AITRL)
Polyclonal Antibody
Prod. No.: G620
Host: Rabbit
Conc.: 0.2 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human GITRL recognizes Human and Mouse GITRL. This polyclonal antibody was purified using affinity chromatography.
Background:
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases (reviewed in 1). The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions.2,3 Its ligand GITRL is expressed in endothelial and antigen-presenting cells4 and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR.5 Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.4
Known Species Cross Reactivity: Ms+, Hu+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse GITRL by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is THP-1 Whole Cell Lysate (Leinco Prod. No.: T206).
References
1. Gaur, U. et al. (2003) Biochem. Pharmacol. 66:1403
2. Ronchetti, S. et al (2002) Blood 100:350
3. Shimizu, J. et al. (2002) Nat. Immunol. 3:135
4. Gurney, A. L. et al. (1999) Curr. Biol. 9:215
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
THP-1 Whole Cell Lysate (Leinco Prod. No.: T206)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
