产品编号:G621
产品描述:Anti-Human GβL
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human G Beta Protein Subunit-like (GBL)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: G621
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human G Beta Protein Subunit-like (GBL) recognizes Human, Mouse and Rat GBL. This polyclonal antibody was purified using affinity chromatography.
Background:
GβL (G protein beta protein subunit-like) is a member of a signaling pathway that regulates mammalian cell growth in response to the presence of nutrients and growth factors.1 It binds to the kinase domain of TOR (Target of rapamycin, also known as mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3). TOR is normally associated with GβL and an additional regulatory protein RAPTOR, allowing TOR to control protein biosynthesis.3 The binding of GβL to TOR stimulates TOR’s kinase activity towards downstream proteins such as RPS6K (ribosomal protein S6 kinase) and the translation factor 4E-BP1 which leads to increased protein translation and cell growth.3
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
G Beta Protein Subunit-like Blocking Peptide (Leinco Prod. No.: G622)
Blocking Peptide
G Beta Protein Subunit-like Blocking Peptide (Leinco Prod. No.: G622)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat GBL by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Brain Tissue Lysate (Leinco Prod. No.: H1407).
References
1. Kim, D. H. et al. (2003) Mol. Cell 11:895
2. Shamji, A. F. et al. (2003) Mol. Cell 12:271
3. Fingar, D. C. et al. (2004) Oncogene 23:3151
Related Products
G Beta Protein Subunit-like Blocking Peptide (Leinco Prod. No.: G622)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Brain Tissue Lysate (Leinco Prod. No.: H1407)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
