产品编号:E184

产品描述:Anti-Human ECSIT(CT)

反应种属:mouse, human

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human ECSIT
Purified No Carrier Protein
(Evolutionarily Conserved Signaling Intermediate in Toll Pathway)
Polyclonal Antibody

Prod. No.: E184
Host:         Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human ECSIT recognizes an epitope near the C-terminus of Human and Mouse ECSIT. This polyclonal antibody was purified using affinity chromatography.

Background:
Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
ECSIT Blocking Peptide (Leinco Prod. No.: E185)
Blocking Peptide
ECSIT Blocking Peptide (Leinco Prod. No.: E185)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse ECSIT by Western blot analysis at a concentration of 0.5 - 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Heart Tissue Lysate (Leinco Prod. No.: H1405).

References
1. Takeda, K. et al. (2003) Annu. Rev. Immunol. 21:335
2. Vogel, S.N. et al. (2003) Mol. Interv. 3:466
3. Janssens, S. et al. (2003) Mol. Cell. 11:293
4. Sato, S. et al. (2003) J. Immunol. 171(8):4304
Related Products
ECSIT Blocking Peptide (Leinco Prod. No.: E185)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Heart Tissue Lysate (Leinco Prod. No.: H1405)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.