产品编号:D245
产品描述:Anti-Human DNase II(CT)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human DNase II
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: D245
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human DNase II recognizes an epitope near the C-terminus of Human DNase II. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus . DNase II causes both chromatin condensation and DNA fragmentation . The genes encoding human,2,4 porcine,4 and murine5 DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12 kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation.3 DNase II is ubiquitously expressed in human tissues.
Known Species Cross Reactivity: Hu+
Immunogen
DNase II (CT) Blocking Peptide (Leinco Prod. No.: D273)
Blocking Peptide
DNase II (CT) Blocking Peptide (Leinco Prod. No.: D273)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human DNase II by Western blot analysis at a dilution of 1:500-1:1,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are THP-1 Whole Cell Lysate (Leinco Prod. No.: T206) or Human Spleen Tissue Lysate (Leinco Prod. No.: H1410).
References
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6
3. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14
4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9
Related Products
DNase II (CT) Blocking Peptide (Leinco Prod. No.: D273)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
THP-1 Whole Cell Lysate (Leinco Prod. No.: T206)
Human Spleen Tissue Lysate (Leinco Prod. No.: H1410)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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