产品编号:D240
产品描述:Anti-Human DFF40(I18)
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Mouse DFF40
Purified No Carrier Protein
(CAD, Caspase Activated Deoxyribonuclease)
Polyclonal Antibody
Prod. No.: D240
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human DFF40 recognizes an epitope near the center of mouse, rat and human DFF40. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively.3,5 DFF45/ICAD is the inhibitory protein of DFF40/CAD1,2 and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
DFF40 (I18) Blocking Peptide (Leinco Prod. No.: D270)
Blocking Peptide
DFF40 (I18) Blocking Peptide (Leinco Prod. No.: D270)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect mouse, rat and human DFF40 by Western blot analysis at a dilution of 1:500-1:1,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are K562 Whole Cell Lysate (Leinco Prod. No.: K128) or Jurkat Whole Cell Lysate (Leinco Prod. No.: J103).
References
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
3. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40
4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
Related Products
DFF40 (I18) Blocking Peptide (Leinco Prod. No.: D270)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
Anti-Human DFF40 (Intermediate Domain) (Leinco Prod. No.: D241)
Anti-Human DFF40 (NT) (Leinco Prod. No.: D242)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
