产品编号:D255
产品描述:Anti-Human DcR3(NT)
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human DcR3
Purified No Carrier Protein
(TR6, Decoy Receptor 3 Precursor)
Polyclonal Antibody
Prod. No.: D255
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human DcR3 recognizes an epitope near the N-terminus of mouse, rat and human DcR3. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. Several novel members in the TNFR family including DR3, DR4, DR5, and DR6 were recently discovered and function as cell death receptors. Two decoy receptors, DcR1 and DcR2, were recently identified to compete with DR4 and DR5 for their ligand TRAIL binding. A novel decoy receptor was more recently discovered and designated DcR3 and TR6, respectively.1,2 Unlike DcR1 and DcR2, DcR3 is a soluble rather than a membrane associated molecule. DcR3 binds to FasL and LIGHT and inhibits FasL and LIGHT induced apoptosis.1,2 DcR3 transcript is expressed in a number of lung and colon carcinomas and in some normal tissues.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
DcR3 (NT) Blocking Peptide (Leinco Prod. No.: D267)
Blocking Peptide
DcR3 (NT) Blocking Peptide (Leinco Prod. No.: D267)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human DcR3 by Western blot analysis at a dilution of 1:500-1:1,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Heart Tissue Lysate (Leinco Prod. No.: H1405).
References
1. Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, Huang A, Donahue CJ, Sherwood SW, Baldwin DT, Godowski PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD, Botstein D, Ashkenazi A. Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 1998;396:699-703
2. Yu KY, Kwon B, Ni J, Zhai Y, Ebner R, Kwon BS. A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis. J Biol Chem 1999;274:13733-6 (RD1299)
Related Products
DcR3 (NT) Blocking Peptide (Leinco Prod. No.: D267)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Heart Tissue Lysate (Leinco Prod. No.: H1405)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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