产品编号:D256
产品描述:Anti-Human DAPK2
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human DAPK2
Purified No Carrier Protein
(Death-Associated Protein Kinase 2)
Polyclonal Antibody
Prod. No.: D256
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human DAPK2 recognizes an epitope near the C-terminus of mouse, rat and human DAPK2. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1.1,2 Ectopicly expressed DAPK2 induced apoptosis in various types of cells.1,2 DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.1
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
DAP kinase 2 Blocking Peptide (Leinco Prod. No.: D261)
Blocking Peptide
DAP kinase 2 Blocking Peptide (Leinco Prod. No.: D261)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect mouse, rat and human DAPK2 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180).
References
1. Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80
2. Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)
Related Products
DAP kinase 2 Blocking Peptide (Leinco Prod. No.: D261)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
