产品编号:C1114

产品描述:Anti-Human Caspase-10(CT)

反应种属:mouse, human, rat

实验方法:WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human Caspase-10 (Casp-10)
Purified No Carrier Protein
(FLICE2, ALPS2)
Polyclonal Antibody

Prod. No.: C1114
Host:         Rabbit
Conc.:       0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human Caspase-10 recognizes an epitope near the C-terminus of mouse, rat and human Caspase-10. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch41,2 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis1. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Caspase-10 (CT) Blocking Peptide (Leinco Prod. No.: C1127)
Blocking Peptide
Caspase-10 (CT) Blocking Peptide (Leinco Prod. No.: C1127)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect mouse, rat and human Caspase-10 by Western blot analysis at a dilution of 1:500-1:1,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).

References
1. Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1β-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 1997;272:6578-6583

2. Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM, Wang L Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. USA. 1996;93:7464-7469

3. Cohen GM. Caspases: the executioners of apoptosis. Biochem J 1997;326:1-16 (RD1299)
Related Products
Caspase-10 (CT) Blocking Peptide (Leinco Prod. No.: C1127)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.