产品编号:C1235
产品描述:Anti-Human Caspase-6(Intermediate Domain)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Caspase-6
Purified No Carrier Protein
(Mch2)
Polyclonal Antibody
Prod. No.: C1235
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Caspase-6 recognizes an epitope in the intermediate domain of Human Caspase-6. This polyclonal antibody was purified using affinity chromatography.
Background:
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens.1,2 The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death.3 Caspase-6 is an executioner caspase that was identified based on its homology with human caspases 2 and 3 as well as the C. elegans cell death protein CED-3.4 It possesses two isoforms, of which only the longer form possesses protease activity.4 Caspase-6 is highly expressed in adult brain and may play a role in several neuronal pathologies.5
Known Species Cross Reactivity: Hu+
Immunogen
Caspase-6 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1273)
Blocking Peptide
Caspase-6 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1273)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human Caspase-6 by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is MCF7 Whole Cell Lysate (Leinco Prod. No.: M1123). Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
References
1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2. Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3. Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4. Fernandes-Alnemri T, Litwack G, and Alnemri ES. Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res. 1995; 55:2737-42.
Related Products
Caspase-6 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1273)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
MCF7 Whole Cell Lysate (Leinco Prod. No.: M1123)
Anti-Human Caspase-6 (NT) (Leinco Prod. No.: C1237)
Caspase-6 (NT) Blocking Peptide (Leinco Prod. No.: C1274)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
