产品编号:C1223
产品描述:Anti-Human Casp-5(Intermediate Domain)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Casp-5
Purified No Carrier Protein
(ICH-3, ICE relIII)
Polyclonal Antibody
Prod. No.: C1223
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Casp-5 recognizes an epitope in the intermediate domain of Human Casp-5. This polyclonal antibody was purified using affinity chromatography.
Background:
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens.1 Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18.2,3 Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β.4
Known Species Cross Reactivity: Hu+
Immunogen
Caspase-5 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1271)
Blocking Peptide
Caspase-5 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1271)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human Casp-5 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Ramos Whole Cell Lysate (Leinco Prod. No.: R1173).
References
1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2. Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
3. Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
4. Martinon F, Burns K, and Tschopp J. The inflammasome: a molecular platform triggering activation of the inflammatory caspases and processing of proIL-1β. Mol. Cell. 2002; 10:417-26.
Related Products
Caspase-5 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1271)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Ramos Whole Cell Lysate (Leinco Prod. No.: R1173)
Anti-Human Casp-5 (NT) (Leinco Prod. No.: C1230)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
