产品编号:B451
产品描述:Anti-Human Beclin-1(CT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Beclin-1
Purified No Carrier Protein
(Coiled-coil myosin-like Bcl-2-interacting protein)
Polyclonal Antibody
Prod. No.: B451
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Beclin-1 recognizes an epitope near the C-terminus of human and mouse Beclin-1. This polyclonal antibody was purified using affinity chromatography.
Background:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells.1,2 Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6,3,4 is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors.5 Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.6
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Beclin-1 (CT) Blocking Peptide (Leinco Prod. No.: B482)
Blocking Peptide
Beclin-1 (CT) Blocking Peptide (Leinco Prod. No.: B482)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human and mouse Beclin-1 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180).
References
1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
4. Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.
Related Products
Beclin-1 (CT) Blocking Peptide (Leinco Prod. No.: B482)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180)
Anti-Human Beclin-1 (NT) (Leinco Prod. No.: B452)
Beclin-1 (NT) Blocking Peptide (Leinco Prod. No.: B483)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
