产品编号:B448
产品描述:Anti-Human Bcl-G
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human Bcl-G
Purified No Carrier Protein
(Bcl-2-like 14)
Polyclonal Antibody
Prod. No.: B448
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Bcl-G recognizes human, mouse and rat Bcl-G. This polyclonal antibody was purified using affinity chromatography.
Background:
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain.1,2 BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G.3 The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.3
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Bcl-G (CT) Blocking Peptide (Leinco Prod. No.: B474)
Blocking Peptide
Bcl-G (CT) Blocking Peptide (Leinco Prod. No.: B474)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect mouse, rat and human Bcl-G by Western blot analysis at a concentration of 2.5-5 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are U937 Whole Cell Lysate (Leinco Prod. No.: U123) and Mouse Testis Tissue Lysate (Leinco Prod. No.: T269).
References
1. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
2. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
3. Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.
Related Products
Bcl-G (CT) Blocking Peptide (Leinco Prod. No.: B474)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
U937 Whole Cell Lysate (Leinco Prod. No.: U123)
Mouse Testis Tissue Lysate (Leinco Prod. No.: T269)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
