产品编号:B384
产品描述:Anti-Human BMF(NT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human Bcl-2 Modifying Factor (BMF)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: B384
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Bcl-2 Modifying Factor (BMF) recognizes and epitope near the N-terminus of human and mouse BMF. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor).1 The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program.1,2 Bmf is constitutively expressed in many tissues.1,2
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Bcl-2 Modifying Factor (NT) Blocking Peptide (Leinco Prod. No.: B495)
Blocking Peptide
Bcl-2 Modifying Factor (NT) Blocking Peptide (Leinco Prod. No.: B495)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human and mouse BMF by Western blot analysis at a concentration of 2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Goat Anti-Armenian Hamster IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: A128).
References
1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001;293(5536):1829-32.
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science. 2001;293(5536):1784-5.
Related Products
Bcl-2 Modifying Factor (NT) Blocking Peptide (Leinco Prod. No.: B495)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Goat Anti-Armenian Hamster IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: A128)
Anti-Human Bcl-2 Modifying Factor (CT) (BMF) (Leinco Prod. No.: B382)
Bcl-2 Modifying Factor (CT) Blocking Peptide (Leinco Prod. No.: B494)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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