产品编号:B446
产品描述:Anti-Human Bcl-2(NT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human Bcl-2
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: B446
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Bcl-2 recognizes an epitope near the N-terminus of human and mouse Bcl-2. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.2 Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis.2,3 These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis.4 It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad.5 Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.4,6
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Bcl-2 (NT) Blocking Peptide (Leinco Prod. No.: B472)
Blocking Peptide
Bcl-2 (NT) Blocking Peptide (Leinco Prod. No.: B472)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentraion of 2 µg/ml. The positive control tissue for IHC is human kidney.
Western Blotting: This antibody can be used to detect Human Bcl-2 by Western blot analysis at a concentration of 1-2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are Daudi Whole Cell Lysate (Leinco Prod. No.: D310) and Human Kidney Tissue Lysate (Leinco Prod. No.: H1409).
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4. Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
Related Products
Bcl-2 (NT) Blocking Peptide (Leinco Prod. No.: B472)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Daudi Whole Cell Lysate (Leinco Prod. No.: D310)
Human Kidney Tissue Lysate (Leinco Prod. No.: H1409)
Bcl-2 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B471)
Anti-Human Bcl-2 (Intermediate Domain) (Leinco Prod. No.: B445)
Anti-Human Bcl-2 (Leinco Prod. No.: B589)
Anti-Human Bcl-2 (Leinco Prod. No.: B590)
Recombinant Human Bcl-2 (minus c-terminal) (Leinco Prod. No.: B548)
Anti-Human/Mouse Bcl-2 ( Antigen Aff. Pur. ) (Leinco Prod. No.: B642)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
