产品编号:B442
产品描述:Anti-Human BAG-1(NT)
反应种属:mouse, human
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human BAG-1
Purified No Carrier Protein
(Bcl-2-binding athanogene-1, receptor-associated protein 46, RAP46)
Polyclonal Antibody
Prod. No.: B442
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human BAG-1 recognizes an epitope near the N-terminus of human and mouse BAG-1. This polyclonal antibody was purified using affinity chromatography.
Background:
Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
BAG-1 (NT) Blocking Peptide (Leinco Prod. No.: B467)
Blocking Peptide
BAG-1 (NT) Blocking Peptide (Leinco Prod. No.: B467)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect human and mouse BAG-1 by Western blot analysis at a concentration of 1-2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PN:P179.
References
1. Takayama S, Sato T, Kraweski K, et al. Cloning and functional analysis of BAG-1: a novel Bcl2-binding protein with anti-cell death activity. Cell 1995; 80:279-84.
2. Nollen EAA, Brunsting JF, Song J, et al. Bag1 functions in vivo as a negative regulator of Hsp70 chaperone activity. Mol. Cell. Biol. 2000; 20:1083-8.
3. Cato ACB and Mink S. BAG-1 family of cochaperones in the modulation of nuclear receptor action. J. Steroid Biochem. & Mol. Biol. 2001; 78:379-88.
4. Kajewska M, Turner BC, Shabaik A, et al. Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers. Prostate 2006; 66:801-10.
Related Products
BAG-1 (NT) Blocking Peptide (Leinco Prod. No.: B467)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PN:P179
Anti-Human BAG-1 (CT) (Leinco Prod. No.: B441)
BAG-1 (CT) Blocking Peptide (Leinco Prod. No.: B466)
Anti-Human Bag-1 (Leinco Prod. No.: B593)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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