产品编号:B480
产品描述:Anti-Human BAP31
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human B Cell Receptor-Associated Protein 31 (BAP31)
Purified No Carrier Protein
(BCR Associated Protein 31)
Polyclonal Antibody
Prod. No.: B480
Host: Rabbit
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human B Cell Receptor-Associated Protein 31 (BAP31) recognizes an epitope in the intermediate domain of Human, Mouse and Rat BAP31. This polyclonal antibody was purified using affinity chromatography.
Background:
Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family.1,2 These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18.3,4 Bap31 may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
B Cell Receptor-Associated Protein 31 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B500)
Blocking Peptide
B Cell Receptor-Associated Protein 31 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B500)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat BAP31 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Rat Lung Tissue Lysate (Leinco Prod. No.: R1010).
References
1. Little, E. et al. (1994) Crit. Rev. Eukaryot. Gene Expr. 4:1
2. Kim, K. M. et al. (1994) EMBO J. 13:3793
3. Paquet, M. E. et al. (2004) J. Immunol. 172:7548
4. Zen, K. et al. (2004) J. Biol. Chem. 279:44924
Related Products
B Cell Receptor-Associated Protein 31 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B500)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Rat Lung Tissue Lysate (Leinco Prod. No.: R1010)
Anti-Human B Cell Receptor-Associated Protein 31 (CT) (BAP31) (Leinco Prod. No.: B479)
B Cell Receptor-Associated Protein 31 (CT) Blocking Peptide (Leinco Prod. No.: B499)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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