产品编号:A253
产品描述:Anti-Human Aven(NT)
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Aven
Purified No Carrier Protein
(Apoptosis caspase activation inhibitor)
Polyclonal Antibody
Prod. No.: A253
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Aven recognizes an epitope near the N-terminus of human, mouse and rat Aven. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9. It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients. Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Aven (NT) Blocking Peptide (Leinco Prod. No.: A267)
Blocking Peptide
Aven (NT) Blocking Peptide (Leinco Prod. No.: A267)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human, mouse and rat Aven by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Raji Whole Cell Lysate (Leinco Prod. No.: R1009).
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Chau BN, Cheng EH-Y, Kerr DA, et al. Aven, a novel inhibitor of caspase activation. Binds Bcl-xL and Apaf-1. Mol. Cell 2000; 6:31-40.
3. Paydas S, Tanriverdi K, Yavuz S, et al. Survivin and aven: two distinct antiapoptotic signals in acute leukemias. Ann. Oncology 2003; 14:1045-50.
Related Products
Aven (NT) Blocking Peptide (Leinco Prod. No.: A267)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Raji Whole Cell Lysate (Leinco Prod. No.: R1009)
Anti-Human Aven (CT) (Leinco Prod. No.: A252)
Aven (CT) Blocking Peptide (Leinco Prod. No.: A266)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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