产品编号:A244
产品描述:Anti-Human AES(NT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Amino-terminal Enhancer of Split (AES)
Purified No Carrier Protein
(GRG, ESP1, TLE5)
Polyclonal Antibody
Prod. No.: A244
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Amino-terminal Enhancer of Split (AES) recognizes an epitope near the N-terminus of human and mouse AES. This polyclonal antibody was purified using affinity chromatography.
Background:
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment.1 Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination.2,3 AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis.4 It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family.4 Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin.4
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Amino-Terminal Enhancer of Split (NT) Blocking Peptide (Leinco Prod. No.: A256)
Blocking Peptide
Amino-Terminal Enhancer of Split (NT) Blocking Peptide (Leinco Prod. No.: A256)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human AES by Western blot analysis at a concentration of 2-4 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is 293 Whole Cell Lysate (Leinco Prod. No.: A182).
References
1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
2. Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.
3. Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.
4. Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.
Related Products
Amino-Terminal Enhancer of Split (NT) Blocking Peptide (Leinco Prod. No.: A256)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
293 Whole Cell Lysate (Leinco Prod. No.: A182)
Anti-Human Amino-terminal Enhancer of Split (CT) (AES) (Leinco Prod. No.: A242)
Amino-Terminal Enhancer of Split (CT) Blocking Peptide (Leinco Prod. No.: A255)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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